A protected 5(6)-Carboxyfluorescein in which the hydroxyl groups are acylated with isobutyric acid. The protection prevents self-condensation during carboxyl activation andavoids the formation of fluorophore-oligomer containg by-products associated with the use of unprotected FAM.
Van der Poel, J.J., et al.: Immunol. Lett., 2, 187 (1981), Fries, J.R., et al.: Chimica Oggi, 19, 18 (2001), Maly, D.J., et al.: Chembiochem., 3, 16 (2002),
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